Pasteur Institute of India
(An Autonomous Institute Under MoHF&W)

RFFIT

Rapid Fluorescent Focus Inhibition Test (RFFIT)

The Pasteur Institute of India, Coonoor carries out the estimation of rabies serum neutralizing antibody content by a method called Rapid Fluorescent Focus Inhibition Test (RFFIT) for rabies vaccine immunized human and other pet animals in order to confirm the production of rabies neutralizing antibodies in the immunized individuals and other pet animals.

By performing this test, we can confirm the immunized person or other pet animals whether they have got minimum protective level of rabies antibodies to protect them against rabies infection and also preventing rabies transmission.

This test is useful to find out the level of rabies neutralizing antibody present in the immunized person, laboratory workers and professionals (doctors), all pet animals and in the manufacturers of equine or human rabies immunoglobulin production units and vaccine manufacturers to perform their field efficacy studies.

How to send the samples

(a) Human Rabies Antibody titre for immunized individual

Collect the blood sample and separate the serum aseptically and transfer the serum sample into a sterile screw capped vial, tightly close the vial put it in a polythene bag. Send it in a thermocol box containing ice coolant.

(b) Dog/Cat Rabies Antibody titre

Collect your dog/cat’s blood sample and separate the serum aseptically and transfer the serum sample into a sterile screw capped vial, tightly close the vial put it in a polythene bag. Send it in a thermocol box containing ice coolant. With the help of veterinary doctor give the following details of your dog/cat:

  • 1. Name of the Animal
  • 2. Species
  • 3. Sex
  • 4. Breed
  • 5. Age/Date of Birth
  • 6. Colour
  • 7. Microchip No. (if available)
  • 8. Date of Last Rabies Vaccination
  • 9. Rabies Vaccine Details (brand & immunization schedule with date)
  • 10. Hospital/Veterinary Doctor where vaccination done
  • 11. Date of Blood Collection
  • 12. Owner’s Name & Address (including phone number and email)
(c) Manufacturers of equine or human rabies immunoglobulin

Send the sera samples in sterile vials tightly closed and sealed with a cello tape. We need to have about 5 ml. of serum per sample. If the sample is hyper immune equine origin or intermediate bulk sample, please inform the expected neutralizing titre value in order to choose the dilution of serum in the test to be included. The samples should be sent in a thermocol box containing ice coolant.

Results:

The results will be sent to the given address within 15-20 days, after the receipt of the sample, by Speed Post. On request, the scanned copies will be sent by email in advance.

Testing Charges:

The cost of testing charge is Rs.3000/- (Rupees three thousand only) per sample. The payment may be made in the form of Demand Draft drawn in favour of “The Director, Pasteur Institute of India” payable at ‘Coonoor’. The Demand Draft should be accompanied along with the parcel.

Address for samples to be sent:

The samples to be packed in good condition, taking into consideration of the breakage/leakage during the transit, and send it with a covering letter stating all details along with the demand draft to:

The Director,
Pasteur Institute of India,
Landmark: Opposite to Sim's Park,
Kotagiri Road,
Coonoor - 643 103,
The Nilgiris,
Tamil Nadu,
India.

The samples may be sent by Speed Post / any courier service available in Coonoor.

Whom to be contacted:

1. Dr. A. Premkumar, Sr.Research Officer & Controlling Officer (QCD) - Ph.94425 33645

2. Dr (Mrs) Sibani Barman, Medical Officer - Ph.0423-2238034

World Health Organization page - https://www.who.int/rabies/human/test_antibody/en/